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Policy Number: 2012-044-v
Policy Title: Counting Zebrafish Usage
Policy: OLAW considers larval and adult forms of fish to be covered by the Public Health Service policy, and both of these forms must be counted. OLAW has made the determination that all stages of zebrafish (Brachydanio or Danio species) development greater than 3 days postfertilization (dpf) must be described in animal-use protocols. Zebrafish from 4 days postfertilization onward are considered animals, and therefore must be counted.

This policy acknowledges that making exact counts of zebrafish larval forms and adults is difficult, and allows knowledgeable investigators to rely on experience and expertise to arrive at estimated counts that accurately reflect numbers of zebrafish 4 dpf and older that are used.

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