Policy Number: 2012-044-v
Policy Title: Counting Zebrafish Usage

Purpose: UW-Madison is responsible to OLAW (Office of Laboratory Animal Welfare) and USDA for assuring that animal numbers on approved protocols are accounted for. This policy is to provide direction to investigators regarding when they must count zebrafish (Brachydanio or Danio species) as part of an animal-use protocol.

POLICY: OLAW considers larval and adult forms of fish to be covered by the Public Health Service policy, and both of these forms must be counted. OLAW has made the determination that all stages of zebrafish development greater than 3 days postfertilization (dpf) must be described in animal-use protocols. Zebrafish from 4 days postfertilization onward are considered animals, and therefore must be counted.

This policy acknowledges that making exact counts of zebrafish larval forms and adults is difficult, and allows knowledgeable investigators to rely on experience and expertise to arrive at estimated counts that accurately reflect numbers of zebrafish 4 dpf and older that are used.

Author: J. Welter, D. Jefcoat
ePublication Date: 05/04/2012
History: Amended 9/6/2013; Reviewed, no changes 9/2017