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SOP for Mixing MS-222

See policy UW-4123, Use of Nonpharmaceutical-Grade Compounds

MS-222 is used for aquatic animals, such as fish and frogs. FINQUEL and Tricaine-S are considered to be pharmaceutical-grade. In the event non-pharmaceutical-grade MS-222 is scientifically justified in an approved protocol, this SOP for mixing can be used.

INGREDIENTS

STOCK SOLUTION

MIXING FROM STOCK SOLUTION

MIXING FOR DIRECT USE

  1. Dissolve MS-222 in tank water
    • Fish (variable by species): 25-300 mg/L (25-100 mg/L = a good starting point for zebrafish)
    • Frogs, salamanders (variable by species): 200-1000 mg/L for anesthesia, 2000 mg/L for euthanasia
  2. Adjust the pH to about 7.4 using sodium bicarbonate
    • Most critical at concentrations above 500 mg/L

NOTES

For surgical purposes, fresh solution should be made for every surgery to minimize contamination and infection

For euthanasia, MS-222 should be made fresh weekly

Discard old MS-222 down the sink diluted with lots of fresh cold water

Avoid exposure to skin; wear gloves while mixing; CAN BE NEUROTOXIC IN HUMANS

2017-09-08

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