SOP for Mixing MS-222
See policy UW-4123, Use of Nonpharmaceutical-Grade Compounds
MS-222 is used for aquatic animals, such as fish and frogs. FINQUEL and Tricaine-S are considered to be pharmaceutical-grade. In the event non-pharmaceutical-grade MS-222 is scientifically justified in an approved protocol, this SOP for mixing can be used.
INGREDIENTS
- MS-222 powder
- Sodium bicarbonate
- pH paper
STOCK SOLUTION
- MS-222 powder can be mixed at 10g/l, using sodium bicarbonate to buffer to a final pH of about 7.4
- Store at -20C; use the expiration date of the powder from the parent bottle as the expiration date of the stock solution
MIXING FROM STOCK SOLUTION
- Dilute stock solution 1:10 for a final dilution of 100 mg/l for surgical anesthesia for fish
- Be sure to check pH after diluting stock solution and adjust to about 7.4 using sodium bicarbonate
MIXING FOR DIRECT USE
- Dissolve MS-222 in tank water
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- Fish (variable by species): 25-300 mg/L (25-100 mg/L = a good starting point for zebrafish)
- Frogs, salamanders (variable by species): 200-1000 mg/L for anesthesia, 2000 mg/L for euthanasia
- Adjust the pH to about 7.4 using sodium bicarbonate
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- Most critical at concentrations above 500 mg/L
NOTES
For surgical purposes, fresh solution should be made for every surgery to minimize contamination and infection
For euthanasia, MS-222 should be made fresh weekly
Discard old MS-222 down the sink diluted with lots of fresh cold water
Avoid exposure to skin; wear gloves while mixing; CAN BE NEUROTOXIC IN HUMANS
2017-09-08